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[111] [112] [113] Invention of Automated Meiotic Mapping [edit] Prior to 2013, despite the development of methods for massively parallel sequencing and their application in finding induced germline mutations, [114] [115] [116] positional cloning remained a slow process, limited by the need to genetically map mutations to chromosomal intervals to ascertain which induced mutation (among the average of approximately 60 changes in coding and splicing function induced per pedigree) was responsible for an observed phenotype

and (4) references cited in identified epidemiologic and animal toxicological secondary reviews

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