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the receiving medium placed in the diffusion cells must be one which mimics the physiological condition of the skin

Timeline depends on the indication

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M.BastianelliS.GiustariniD.PierucciS.BustiC.et al

The 2016 Draelos study in Journal of Drugs in Dermatology compared a crosslinked HA serum with recombinant EGF against a fibroblast-conditioned media serum and documented meaningful improvements in skin appearance and texture [31]