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8-OHdG expression and apoptotic cell death were assessed using immunohistochemical techniques

As the primary organ responsible for steroid metabolism, the liver is particularly susceptible to the toxic effects of 17--alkylated oral anabolicandrogenic steroids, such as stanozolol and methyltestosterone ( Mechanistic studies suggest that anabolicandrogenic steroids impair mitochondrial -oxidation and deplete intracellular glutathione stores, resulting in lipid peroxidation and activation of apoptotic signalling pathways

Measures included MPS, albumin protein synthesis (APS), plasma amino acids/insulin, leg/muscle blood flow, and anabolic signaling using isotope tracers and phosphoimmunoblotting

doi:10.1073/pnas.1002178107, PMID: 20713697 Adam-Vizi V, Chinopoulos C (2006) Bioenergetics and the formation of mitochondrial reactive oxygen species

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Eur J Med Chem 174:87115