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After 2448 h, the cells were collected for RNA and protein extraction

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Reactive oxygen species (ROS) levels were assessed by C11-BODIPY probe, oxidative stress indicators including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase were measured by kits, and Cytochrome C, Ferritin, mitochondrial transcription factor A (TFAM), glutathione peroxidase 4 (GPX4) and SLC7A5 expression were detected by Western blot

Thus, there is an urgent need to identify a marker of structural damage to the kidney before functional impairment and the vicious cycle of inflammation, microcirculatory disturbances, and local ischemia are established (116)