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Secystathionine could then be changed into selenohomocysteine (SeHCys) via cysthathionine--lyase (Figure 2, step 6)

Researchers use LC-MS to verify: .Molecular weight .Peptide identity .Structural integrity .Manufacturing accuracy Combining HPLC with LC-MS provides a comprehensive assessment of peptide quality

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The eluates were subjected to SDS-PAGE and visualized by Western blotting using antibodies against CYP1A2, CYP3A4 (Santa Cruz: sc-30085 and sc-53850, respectively), FLAG and EGFR (Cell signaling: #2232S)