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primarily rough ER
particularly the diverse array of proteins is crucial for research in blood biology and disease
in some applications such as DNA and RNA extraction
The protocol can be completed in about one hour
A Dounce homogenizer or a tissue blender is not needed
MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit 184475-35-2 primarily rough ERThe kit is designed for rapid fractionation of fresh frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100 200 mg starting material. By using a specialized filter cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol, nuclei, chloroplasts and organelles) can
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