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Some studies cited in a 2025 review noted that while proline can protect cells at low levels, when metabolized by the enzyme proline oxidase it produces reactive oxygen species (ROS)

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[DOI] [PubMed] [Google Scholar] 6.Barisic I., Balenovic D., Klicek R., Radic B., Nikitovic B., Drmic D., Udovicic M., Strinic D., Bardak D., Berkopic L., et al

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Methionine is an essential amino acid that must be supplemented through ones diet