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In the context of mainstream smoke production, the connection between the smouldering and puffing processes through the interpuff period was intimately linked

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Nicotine C-oxidation to nicotine 5(1)iminium ion is followed by a second oxidation that generates cotinine, which is further metabolized to trans 3-hydroxycotinine (3-HCOT), a reaction that is catalyzed almost exclusively by CYP2A6 (see Fig