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Three of the genome-wide significant loci have known associations with ND from our prior GWAS and others 6 : chr15q25 21,22,23 (smallest P = 1.6 10 39 for rs16969968, a well-established functional missense [D398N] CHRNA5 SNP 24 ) chr20q13 21 (smallest P = 1.2 10 12 for rs151176846, an intronic CHRNA4 SNP), and chr9q34 22 (smallest P = 1.1 10 8 for rs13284520, an intronic DBH SNP)

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