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Specifically, the addition of cuprizone (bis-cyclohexanone oxalydihydrazone), to the diet process (66) produces a massive demyelination in different areas of the CNS and particularly in the corpus callosum (67-69)

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coli strain BL21 as previously described 23

But with a 2mg/mL concentration (5mL of water added), that same 4mg dose requires 2.0mL of liquid, which is an entire insulin syringe worth and creates a large, uncomfortable injection volume