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The method described in that paper is now known as the Zimm plot, which allows determination of M w , R g , and second virial coefficient ( A 2 ) of a macromolecule in a solution by measuring light scattering across various angles and concentrations

They were then treated for 2 h with a 0.1 M sodium cacodylate buffer containing 2% OsO 4 , followed by an overnight treatment with 1% aqueous uranyl acetate for 18 h

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Autophagy 2020;16:317