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Immunophenotyping Human peripheral blood mononuclear cells (PBMCs) were isolated from heparinized whole blood by density gradient centrifugation with Lymphoprep (StemCell Technologies, Vancouver, BC) and immunophenotyped as previously described 21

Notably, cigarette smoke exposure induced significant shifts in the intestinal microbiome, notably increasing Akkermansiaceae abundance, which was reversed upon cessation

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