3. Structural support processes
This is not just a theoretical issue
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Lyophilized powder in the freezer tolerates brief thaws (under 4 hours) if immediately re-frozen, but repeated freeze-thaw cycles degrade potency by 510% per cycle
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BALB/c nude mice were randomized into two groups and the number of each group was 7 and the sample size was estimated from preliminary experiments or from reports in the literature