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Always remember to remove air bubbles from the pin by flicking it gently, pin side up, and pushing bubbles out

As a precursor for neurotransmitters like glutamate and GABA, glutathione is essential for brain function

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The tissue was incubated at 37 C on a shaking incubator set at 250 rpm for 1 h, with the centrifuge tube being gently inverted every 20 min

\(\alpha \)-Tubulin was used as the loading control

Discussion Deficiency of vitamin D (VD) is a significant and most common risk factor for chronic diseases, including insulin resistance, obesity, diabetes, and atherosclerosis 20,21,22,23