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Quantitative real-time PCR (qRT-PCR) Total RNA was isolated from pancreatic tissues and cultured PSCs using TRIzol reagent (Beyotime)

Astrocytes give rise to new neurons in the adult mammalian hippocampus

Cell lysates were passed through 27-G needle and the extracts were centrifuged for 15 min at 15,000 g

262 In summary, Met restriction (MR) can alleviate diabetes by interfering with glucose homeostasis, increasing insulin sensitivity and inflammatory response

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