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The pellet was resuspended in 0.1 M K 2 P 2 O 7 , 1 mmol/L EDTA (pH 7.4) and centrifuged again for 65 min at 105,000 g to give the final microsomal fraction

Exposomes and biodosimetry illustrate the multidisciplinary approach necessary to thoroughly assess astronaut health

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10.1007/s10439-016-1752-x 70 WolfS.DeuelJ

Serum limitation-induced organ culture model of keloids and apoptotic induction of FOXO4-DRI in situ The keloid tissues were cut into 4-mm diameter cylinders using a corneal trephine for organ culture and cultured in a DMEM containing 2% FBS for 10 days to construct the serum limitation-induced senescent organ culture model of keloid tissues