[DOI] [PubMed] [Google Scholar] [5].Guillermo Ruiz-Irastorza, George Bertsias
TRPA1 is activated by GSH-sensitive molecules such as ROS and inactivated by GSH [30], but the decreased expression of TRPA1 due to GSH was surprisingly proved in this study
Furthermore, these compounds stabilize enzyme protein conformations, upregulate the transcription and expression of SOD, CAT, and GSH-Px genes, and enhance enzymatic activity to eliminate ROS
Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)
Cytosolic phospholipase A2alpha-deficient mice are resistant to collagen-induced arthritis
The Nrf2/ARE pathway, which controls redox homeostasis and fights against ferroptosis, is another regulatory component