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Cdt 19 (9), 10681076

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at 37C in the presence of Zombie Red (1/1000) according to the manufacturers procedure (Ozyme) then trypsinized and fixed in PBS with formalin 3% (Sigma Aldrich)

These site-specific conjugations theoretically provide many advantages in terms of structural stability, homogeneity, and well-controlled DAR (predominant at 4 in the case of dibromomaleimide) (Behrens et al., 2015)

Signs your dose needs adjustment Increase dose consideration: Minimal response after 3-4 weeks at current dose, stable weight and measurements despite consistent protocol adherence, absence of any subjective improvements in energy or fasted tolerance