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The mobile phase A was 97% water and 3% ACN (20 mM NH 4 Ac, 15 mM NH 4 OH, pH = 9.0), and the mobile phase B was 100% ACN

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Preclinical studies confirm that targeting HSP90 effectively ameliorates depressive-like behaviors: quercetin inhibits HSP90 to suppress NLRP3 inflammasome activation and levels of cytokines (IL-6, IL-1, and TNF-), alleviating hippocampal neuroinflammation [30]

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