The data were categorized and visualized using R packages pheatmap (v1.0.12) ( and ggplot2 (v3.3.3) ( The proteinprotein interaction network among NtTBL proteins in tobacco was constructed using the STRING database (v12) [38] ( The psRNATarget database was utilized to explore the regulatory relationships between micro RNAs (miRNAs) and tobacco TBL genes [39] ( In addition, the PlantTFDB (v5.0) database was employed to investigate the regulatory relationships between transcription factors (TFs) and tobacco TBL genes [40] ( The interaction networks were visualized using Cytoscape (v3.8.1) [41]
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The Chesterfield cigarette brand, owned by Philip Morris International (formerly under Liggett & Myers American Tobacco), carries a rich legacy dating back to the 1940s and 1950s endorsed by icons like Bing Crosby, Bob Hope, Perry Como, Ronald Reagan, and Paul Douglas, and sponsored across NBC radio programs
