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Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

Chilling injury resulting from LT stress is associated with disparity between making and elimination of ROS

This is part of the "copper uglies" phenomenon

It's this whole shell game, but it sets precedent like we covered before

Proc Natl Acad Sci 97(15):84848489 Haviv F et al (2005) Thrombospondin-1 mimetic peptide inhibitors of angiogenesis and tumor growth: design, synthesis, and optimization of pharmacokinetics and biological activities

In one of the largest high-level studies to date comparing hip arthroscopy to physical therapy, there was no difference between the outcomes of the two groups (7)