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Notably, similar inhibitions were observed both with the wild type enzyme and its cysteine mutants, suggesting that thiol conjugation is dispensable for GST inactivation by auronofin in contrast to other described targets 103

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Louis, MO, USA) as well as a single control incubated with the reducing agent -mercaptoethanol (Thermo Scientific, Stockholm, Sweden)

22 HallmayerJ.ClevelandS.TorresA.PhillipsJ.CohenB.TorigoeT.et al

Citrate synthase activity Tissues were homogenized with an electric potter in 50 mM phosphate buffer (pH, 7.5) at 4C and centrifuged at 600 g for 10 minutes, in the presence of protease inhibitor (1:100)

Chemoenzymatic synthesis of cryptophycin anticancer agents by an ester bond-forming non-ribosomal peptide synthetase module