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Unlike growth hormonerelated peptides that act via the pituitary, TB500 works directly at the site of injury to stimulate healing across a wide range of tissuesincluding tendons, muscles, fascia, ligaments, and even cardiac tissue

NAD+s role in cellular repair has also made it a popular experimental therapy for improved health in aging, with the idea that maintaining higher NAD+ might support healthy aging processes

The histological analysis was performed using a high power objective (x63) in the Axioskop 2-Mot Plus Zeiss microscope (Carl Zeiss, Jena, Germany)

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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate