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The regions youth also smoke at higher rates compared to the average U.S

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[8] By October 2023, 54.4% of adult cigarette smokers in England mainly or exclusively smoked hand-rolled cigarettes

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Total RNA Kit (OMEGA Bio-tek, Norcross, GA, USA) and cDNA was made with the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA)