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The supernatant was also saved (tube 2) and the loosened epithelium was then manually scraped off into stock solution with 10% FBS (tube 3), and all cells were collected by centrifugation for 10 min at 225 g , 4 C (tubes 1, 2, and 3)

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19341956] Format: Photographs Genre: Film negatives Location: Boston Public Library Arts Department Collection (local): Leslie Jones Collection Series: Animals: Miscellaneous Subjects: Fish Sunglasses Cigarettes Extent: 1 negative : film, black & white

Vascular endothelium cell dysfunction, which is regarded as the pathological basis of CVDs, is a central or exacerbating component in smoking/vaping-related pathologies