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It has been previously suggested that the accumulation of GSSG may exert cytotoxic effects due to its propensity to form disulfides with intracellular proteins, which leads to their inactivation (Forman et al., 2009

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The GSH- o -phthalaldehyde adducts were separated on a 4.6250 mm Luna C18 column (5 m, Phenomenex, Torrance, CA, U.S.A.) using two Waters 510 pumps, a 717 autosampler, and a 474 fluorescence detector (Milford, MA, U.S.A.), and fluorescence was detected at 420 nm with excitation at 340 nm

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Since it is a water-soluble vitamin and your body uses just what it needs at that time and eliminates the excess through the bodys normal waste process, it cannot build up in your system

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