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The stability of NAD + , NADH, NADP + and NADPH in extraction conditions was verified on solutions of pure compounds using UV-Vis spectroscopy (Fig

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After quantification, -mercaptoethanol (1:100 v/v) and bromophenol blue powder were added

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For instance, the expression of specific metabolic enzymes in tumors may indicate sensitivity to amino acid restriction, while circulating metabolite profiles and microbiome characteristics hold promise as dynamic biomarkers for monitoring the efficacy of nutritional interventions [256, 303]