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To generate Gpx4 -/- and Acsl4 -/- clones by CRISPR Cas9 gene editing, transfection was performed for 48 h followed by puromycin (Gibco, A1113803) selection for 10 days and subsequent seeding onto 96-well plates with one cell per well for expansion

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Since aging is a primary risk factor in neurodegenerative diseases, it is likely that the age-dependent reduction in microbial species diversity, including alterations in relative abundances of region-specific microbes, further facilitates persistent degradation and damage of gut mucosal and gut epithelial layers and increases infiltrations of bacterial toxins (Qin et al., 2007

Chang, Y

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Not a significant source of fat, cholesterol, sugar, or protein