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Samples were withdrawn after 0, 2, 4, 24, 48 and 72 hours and incubated with enzyme and detection reagent for 10 minutes before detection using a SpectraMax i3 plate reader at 550 nm excitation and 595 nm emission

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The blend is administered via subcutaneous injection, typically before bed to align with natural GH secretion patterns

In this study, we prepared Lip-Sil using a lipid composition comprising HSPC, cholesterol, and mPEG2000-DSPE, which is similar to FDA-approved liposomal formulations such as Doxil and AmbiSome 27