Isolation of exosomes Mouse granulosa cells were acclimated in DMEM/F12 without exosomes for 24 h, and then subjected to different interventions
The study reported changes in melatonin-metabolite excretion and selected clock-gene expression measurements
Young RE, Huh DD (2021) Organ-on-a-chip technology for the study of the female reproductive system
Subsequently, protein A or G agarose that had been pre-cleaned twice with NP-40 lysis buffer was added to the mixture and allowed to incubate for an additional 4 h at 4 C
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However, validation of these markers against the gold standard, i.e., Ussing chambers, is still lacking