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GHK-Cu regulates inflammatory molecules TNF- and IL-6, with observed effects on fibrotic remodeling and collagen deposition in the lungs

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10.15406/mojfpt.2018.06.00144 14 BaekM.DiMaioF.AnishchenkoI.DauparasJ.OvchinnikovS.LeeG

For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)