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The adduct was further concentrated, and excess TRaQ-G ligand was removed by desalting with Zeba spin desalting columns (7k molecular weight cutoff, 0.5 ml)

Also, why leave the patient perhaps 2 weeks without their treatment when they need it

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These results suggest that USP33 likely locally, rather than globally, deubiquitinates arrestins associated with D 3 R/Mdm2