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Similarly, 10M of BAY 43-9006 significantly induced hemolysis exclusively in Ringer at 0.4% hematocrit ( p =0.0012), but not in Ringer at 4% hematocrit or in cRPMI at 4% hematocrit

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Protein isolation and western blotting For standard western blotting, tissues were lysed in RIPA buffer and extracted protein content was determined using the BCA protein assay (Sigma, St

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Leaving vials in direct light or heat A sunny counter or a car interior can push a vial well past safe storage temperatures within hours

Butterfield, D