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To reduce the effect of DMSO on living cells, its solutions were diluted in 0.86% NaCl in a ratio of 1:9 (100 L DMSO and 900 L 0.86% NaCl)
The other half was only given levothyroxine and a placebo
Studies demonstrate that LPS activates adipose tissue macrophages via TLR4 receptors, promoting the release of proinflammatory cytokines such as TNF-, which in turn induces excessive lipolysis in adipocytes, generating large quantities of free fatty acids (FFAs) ( 3.5 Hypothalamic-pituitary-ovarian axis disruption Disruption of gut microbiota leading to systemic LPS translocation not only impairs peripheral organ function but also exacerbates PCOS pathology by interfering with the neuroendocrine regulation of the HPO axis ( 4 Gut microbiota characteristics in PCOS Recent studies have shown distinct differences in gut microbial composition between PCOS and healthy controls
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10.3892/mmr.2012.971 Mol
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