Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations
The deproteinized samples were incubated with necessary reaction components for 30 min at room temperature protected from light
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(PubMed) Horev-Azaria L, Eliav S, Izigov N, et al
Common Vial Sizes and Concentrations Vial size 50 mg BAC water 2.0 mL Concentration 25.0 mg/mL 1 mg draw (U-100) 0.04 mL = 4 units Vial size 50 mg BAC water 2.5 mL Concentration 20.0 mg/mL 1 mg draw (U-100) 0.05 mL = 5 units Vial size 100 mg BAC water 3.0 mL Concentration 33.3 mg/mL 1 mg draw (U-100) 0.03 mL = 3 units Vial size 100 mg BAC water 5.0 mL Concentration 20.0 mg/mL 1 mg draw (U-100) 0.05 mL = 5 units Pick a reconstitution volume that produces a draw you can read cleanly on a U-100 syringe
The beneficial effects of astaxanthin on glucose metabolism and modified low-density lipoprotein in healthy volunteers and subjects with prediabetes
