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After three PBS washes, iHLOs were incubated in 100 L Ce3D permeabilization/blocking buffer (BioLegend, catalog: 427706) for 1 h at room temperature

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DENSITIN ( CALCIUM 250 MG + MAGNESIUM 83.3 MG + VITAMIN C 33.33 MG + BORON 0.5 MG + VITAMIN D 3