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Detailed analysis of atpenin A5 and phenformin cytotoxicity and inhibition of metabolic activity in various cell lines As predicted from our modeling approach as tier 1 targets, atpenin A5 at 20 M and phenformin at 100 M do not show toxicity in endpoint measurements based on total cell counting when Calu-3, CaCo-2, A549, and Huh7.5 cells were exposed to the compounds for 72 h (Fig

This concept has widespread support 4,5 and explains animal injury models of disc degeneration 5,6,7

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Therefore, the conclusions drawn from this review are limited by the quality and quantity of the studies included in the analysis