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The next day, cells were pre-treated with phenformin, SR13800, and scyllo-inositol in concentrations of 50 M, 10 M, and 2 M, and atpenin A5 in concentrations of 10 M, 2 M, and 0.4 M

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Depending on the extent of endplate fracture and NP herniation the damage ranges from minor when only small fracture lines are evident to a virtual loss of the entire nucleus tissue to the vertebral bone

For each tested amplicon, a No Template Control (NTC) test was performed, in which no DNA was added (analysis of primer amplification) - to check the amplification performance of the primers