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It was difficult to find people who would agree to be transfused, but an eccentric scholar, Arthur Coga, consented and the procedure was carried out by Lower and King before the Royal Society on 23 November 1667

20 g of propidium iodide (PI) was added for 30 min and microscopic images were taken using Keyence Fluorescent Microscope BZ-X800

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Collectively, these findings imply a role of NLRP3 inflammasome in myocardial infarction injury, and inhibiting NLRP3 inflammasome activation may be a novel therapeutic target for the treatment of myocardial infarction injury