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BPC-157 has extensive animal data for both gastrointestinal and neurological effects but lacks published large-scale human clinical trials for depression specifically

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Employing the following formula prescribed for parallel trials and accounting for a 10% attrition rate in each group, the calculations indicated a need for 22 participants in each group to adequately address the research objectives

On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin