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Dimerization of the N-terminal amphipathic -helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis

We pretreated HepG2 cells with diamide, a well-established protein S -glutathionylation induction agent, for 2 h, followed by TNF stimulation

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It does not affect your measured volume (the air was drawn after measurement) and it reduces dead space waste by pushing the last bit of solution through the needle