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Hydrops fetalis associated with erythrocyte G-6-PD deficiency and maternal ingestion of fava beans and ascorbic acid

LO2 and HepG2 cells were cultured in RPMI 1640 with 10% FBS at 37 in 5% CO 2

Protein samples were mixed with 5 protein loading buffer to a final concentration of 1 and then boiled at 95 C for 10 min, resolved on 12% BisTris SDSPAGE gels and analyzed using a standard immunoblotting protocol

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R The enzyme family responsible is the uridine-diphosphate-glucuronosyltransferases (UGTs) , which transfer glucuronic acid from the cofactor UDP-glucuronic acid (UDPGA) to the substrate