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Fig.2 GST tag resin GST resin technical parameters Buffer recommendation Equilibration buffer: 10-20mM PB/50-100mM Tris-HCl + 0.14-0.4M NaClpH6.2-8.0 Elution buffer50mM Tris-HCl+10-60mM reduced glutathione, pH8.0-9.0 Tips: To improve purity of target molecules, add reducing agent in binding buffer and elution buffer, i.e

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[DOI] [PMC free article] [PubMed] [Google Scholar] [63].Santoro F, Nuez-Gil IJ, Vitale E, Viana-Llamas MC, Reche-Martinez B, Romero-Pareja R, et al

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