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Both these two complexes, independently of each other, use the lipid soluble carrier located into the IMM, ubiquinone (coenzyme Q) to form ubiquinol

GFP or HA tagged GRASP55 and GRASP65-GFP were generated by PCR from GRASP55 or GRASP65 cDNA and digested with XhoI and BamHI or HindIII and KpnI respectively, then inserted into pEGFP-N1 or pHA-N1 vector

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Multi-ligand strategies and optimized surface chemistry further enhance transport efficiency, offering a pathway toward clinically relevant brain-targeted delivery systems