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120,000 in 0.1 M Tris buffer with 5% donkey serum and 0.1% Triton X-100) and developed with a Vectastain HRP kit (Vector Labs, Burlingame, CA) and DAB (Metal Enhanced DAB substrate kit, Thermo Scientific, Rockford, Il)

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36687-82-8 Molecular Weight 472.48 Formula C 18 H 36 N 2 O 12 SMILES C[N+](C)(C)C[C@@H](CC(=O)[O-])O.C[N+](C)(C)C[C@@H](CC(=O)[O-])O.[C@@H]([C@H](C(=O)O)O)(C(=O)O)O Shipping Room temperature in continental US

Heath H & Wildy J (1957) Biosynthesis of ergothioneine