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The 7-day mortality rate and daily mouse weights were tracked, and the LD 50 of CGA injection was determined as previously described (Schlede et al., 1992)

10.1111/bjd.20831 Br J Dermatol

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Taken together, this study provides compelling evidence that Epitalon at a concentration of 0.1 mM effectively mitigates multiple detrimental effects associated with post-ovulatory oocyte aging in vitro

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For measuring GPx (EC 1.11.1.9) activity, the mixture had 1 ml of phosphate buffer (50 mM KPO 4 buffer pH 7.0 + 0.1% Triton X-100), 0.1 ml of 2.4 M NADPH, 12 M GSH, 6 U GR and 0.1 ml of hydrogen peroxide