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After three washes with PB supplemented with 0.3% Triton X-100 (PBT), the sections were incubated overnight at 4C in PBT containing 0.3% BSA (Santa Cruz), 2% normal horse serum (Vector Laboratories), and the mouse anti-NeuN and rabbit anti-TrkA or anti-NGF primary antibodies (at a dilution of 1:200 in all cases except for NeuN, which was used at a dilution of 1:600)

The extensive intracellular network of cell death-regulating factors implies an elaborate and refined multi-level regulation of cell death signaling

Events with amplitude 99% recovery of all proteins from the lysate

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Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T