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Far-UV CD was performed on a Chirascan CD spectrometer (Applied Photophysics Ltd., Leatherhead, UK) between 200 and 260 nm by using a 1-mm path length cell at 293 K

A recurring technical pitfall is that GSH oxidizes rapidly to GSSG during sample handling, so studies that fail to properly stabilize and derivatize samples can artificially inflate the apparent oxidized fraction a source of noise across the literature

As one of the strongest and most efficient antioxidants, glutathione is responsible for eliminating free radicals

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