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Three technical replicates of 100 L culture were taken from each culture flask sample prior to quenching cells in cold methanol/water
The best practice is to withdraw your required dose and immediately return the vial to the refrigerator
Lipid peroxidation was assessed by measuring MDA levels using a Lipid Peroxidation Assay kit (ab118970, Abcam) according to the manufacturer's instructions, with absorbance measured at 532 nm on a microplate reader (Tecan, Switzerland)
Maintain 7 to 9 hours of sleep per night to support hormonal balance and cellular repair processes
Though apparently these results might contradict the results obtained in HT22 cells, the outcomes suggest that it is under a low DHA diet with limited availability of n-3 precursors, when it is especially important to activate mechanisms (like upregulation of Gpx4 isoform expression) that may ensure the preservation of DHA in brain membranes
