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Throughout the experiment, animals were maintained under anaesthesia, and artificial cerebrospinal fluid (NaH 2 PO 4 2.0 mM, MgCl 2 1.0 mM, CaCl 2 1.2 mM, KCl 2.7 mM and NaCl 145 mM) was constantly pumped through the microdialysis probe at a rate of 0.5 L/min

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For most peptide reconstitution, bacteriostatic water is the right choice but the best answer depends on how quickly you plan to use the vial and whether the specific peptide has any known sensitivity to benzyl alcohol

T3 (the active thyroid hormone) production can decrease during prolonged dieting as a metabolic adaptation